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投稿时间：2020-09-04
投稿时间：2020-09-04
中文摘要: 采用超声辅助双水相系统进行槐米总黄酮的提取，以萃取率为指标，考察了聚乙二醇1000（polyethylene glycol 1000，PEG1000）质量分数、料液比、浸提温度和硫酸铵质量分数对总黄酮提取的影响。通过正交试验得出最佳提取工艺参数为聚乙二醇1000质量分数28%，硫酸铵质量分数10%，料液比1∶60（g/mL），浸提温度40℃。在此工艺条件下进行3次平行试验，总黄酮的萃取率为95.91%，得率为20.53 mg/g。该提取方法工艺稳定，结果重现性好。
Abstract:In the study，the extraction process of flavonoids from Sophora japonica L.was investigated using an ultrasound-assisted aqueous two-phase system.The effects of the polyethylene glycol 1000（PEG1000）concentration，solid-liquid ratio，extraction temperature，and ammonium sulfate concentration on the extraction ratio have been investigated by an orthogonal experimental method.The results showed that the optimal extraction conditions were a PEG 1000 and ammonium sulfate concentration of 28% and 10%，respectively，a solid-liquid ratio of 1∶60（g/mL），extraction temperature 40 ℃.Three parallel tests were carried out under these conditions.The extraction rate was 95.91% and the yield was 20.53 mg/g.The process was stable and the results were reproducible.
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