Abstract:Hydroxyl radical（·OH）and superoxide anion radical（O2-·）scavenging rates were used as the main indexes to obtain the collagenase hydrolysate of carp（Cyprinus carpio）scales with high antioxidant activity.The effects of enzymatic hydrolysis temperature，pH，enzyme dosage，substrate concentration，and hydrolysis time on the antioxidant activity and degree of hydrolysis of the hydrolysates were investigated through a single-factor experiment.The process conditions of enzymatic hydrolysis were optimized through response surface analysis.The results showed that the optimum conditions were as follows：the enzymolysis time was 4.4 h，enzymolysis temperature was 66.0 ℃，pH was 8.5，substrate concentration was 20 mg/mL，and enzyme amount was 4%.Under these conditions，the scavenging rates of the·OH，O2-·，and Fe2＋ chelating ability were 80.24%，65.34%，and 59.73%，respectively.Compared to widely used antioxidants，the enzymolysis solution was lower than butylated hydroxyanisole（BHA）and ascorbic acid in terms of the O2-·scavenging ability（P＜0.05）but reached the level of the BHA and ascorbic acid in the·OH scavenging ability （P＞0.05）.In terms of the Fe2＋chelating ability，the enzymatic hydrolysates were significantly better than the BHA and ascorbic acid（P＜0.05）.