Abstract:An insomnia mice model was established through the intraperitoneal injection of p-chlorophenylalanine（PCPA）to explore the therapeutic effect of queen bee larvae on insomnia.Insomnia mice were given either a low（0.1 g/kg），medium（1 g/kg），or high dose（10 g/kg）of lyophilized queen bee larva powder for 7 days.The reliability of the insomnia model and the therapeutic effectiveness of queen bee larva powder were assessed by monitoring behavioral changes and 5-hydroxytryptamine（5-HT）content measurements in mice serum，respectively.The behavior of mice confirmed the effectiveness of the insomnia model and reliably recapitulated the characteristics of insomnia after intraperitoneal injection of PCPA.Furthermore，results showed that the relative bodyweight content of 5-HT in the serum of treated mice was significantly lower than in the insomnia-free control group （P＜0.05）.The content of 5-HT in the serum of queen bee larva powder-treated insomnia mice was significantly higher（P＜0.05）than in the untreated insomnia mice.The insomnia-characteristic behavior disappeared in treated insomnia mice，unlike in the untreated insomnia mice group.Furthermore，there was no significant difference between queen bee larva powder-treated insomnia mice and the insomnia-free control group.