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投稿时间：2020-08-17
投稿时间：2020-08-17
中文摘要: 南极磷虾油中卵磷脂（lecithin，PC）型二十碳五烯酸（ecosapentaenoic acid，EPA）相比于非PC型EPA具有更高的生物活性和生物利用度。为鉴定南极磷虾油中PC型EPA的分布比例，通过薄层色谱法分离南极磷虾油中的PC，利用气相色谱方法对其中的EPA进行定量。测定6种不同来源南极磷虾油中PC型EPA的含量，利用南极磷虾油PC型EPA含量占总EPA含量的比例这一参数评价南极磷虾油的品质。研究结果表明，二十碳五烯酸甲酯（methyl ecosapentaenoic acid，EPA-ME）相比于内标十七烷酸甲酯（methyl heptadecanoate，C17:0-ME）的相对质量校正因子为1.52；所筛选出的优质南极磷虾油的PC型EPA含量占总EPA含量的比例在60%以上，最高可达70%。
Abstract:Lecithin（PC）associated ecosapentaenoic acid（EPA）from Antarctic krill has higher biological activity and bioavailability than non-PC associated EPA.To quantify the distribution ratio of PC-associated EPA in krill oil，the PC in Antarctic krill oil was isolated by thin-layer chromatography and its EPA was quantified by gas chromatography.The content of PC-associated EPA in six kinds of Antarctic krill oil from different sources was accurately determined.Antarctic krill oil quality was evaluated using the ratio of PC-associated EPA to total EPA.The relative mass correction factor for methyl ecosapentaenoic acid （EPA-ME）was 1.52，compared with the internal standard，methyl heptadecanoate（C17:0-ME）.The ratio of PC-associated EPA to total EPA in high quality Antarctic krill oil ranged from＞60% to 70%.
keywords: Antarctic krill oil phospholipids phosphatidylcholine eicosapentaenoic acid quality evaluation
文章编号：202115025 中图分类号： 文献标志码：
基金项目:吉林省科技发展计划项目（20200708050YY）；中国博士后科学基金资助项目（2020M670806）
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