Abstract:In this study，the extraction of high-intensity pulsed electric field-coupled compound enzyme of Brasenia schreberi polysaccharide （BSP）was investigated.The extraction rate was used as the index，and the optimal process parameters were determined through single-factor and orthogonal tests.The results showed that the optimum technological parameters of high-intensity pulsed electric field extraction were as follows:solidliquid ratio 1∶30（g/mL），extraction time 40 min，electric field intensity 25 kV/cm，and number of pulses 6.Under these conditions，the BSP extraction rate was 10.75%.After high-intensity pulsed electric field extraction，compound enzyme extraction was performed.The optimum technological parameters for enzymatic hydrolysis were as follows:compound enzyme addition（papain∶cellulose enzyme∶pectinase 1∶1∶1，mass ratio）2.5%，enzymolysis pH 4.5，enzymolysis time 60 min，and enzymolysis temperature 40 ℃，Under these conditions，the BSP extraction rate was 12.27%.Compared to the single high-intensity pulsed electric field extraction，the extraction rate of polysaccharides from B.schreberi using the high-intensity pulsed electric field-coupled compound enzyme method significantly improved，and the proposed method is suitable for extracting polysaccharides from B.schreberi.