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食品研究与开发:2021,42(15):92-96
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超声波辅助酶法提取紫薯花青素及抗氧化性研究
(许昌学院食品与药学院,河南省食品安全生物标识快检技术重点实验室,河南 许昌 461000)
Ultrasonic-assisted Enzymatic Method Extraction Technology and Antioxidant Activity of Anthocyanins from Purple Potato
(Food and Pharmacy College,Key Laboratory of Biomarker Based Rapid-detection Technology for Food Safety of Henan Province,Xuchang University,Xuchang 461000,Henan,China)
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投稿时间:2020-07-22    
中文摘要: 采用超声波辅助纤维素酶和α-淀粉酶对紫薯花青素进行提取优化。在纤维素酶与α-淀粉酶的质量比为1∶1时,得到提取紫薯花青素的最佳工艺为料液比1∶30(g/mL),酶用量4 mg/g,提取时间40 min,提取温度55℃。对紫薯花青素清除羟自由基能力进行研究,结果表明:紫薯花青素具有较强的自由基清除能力,当浓度为5 mg/mL时,自由基清除率接近100%,与抗坏血酸抗氧化能力相当。
中文关键词: 紫薯  花青素  超声波  提取  抗氧化
Abstract:The ultrasonic-assisted enzymatic method was used to extract the anthocyanin from purple potato in order to increase the yield of anthocyanins.Under the condition the mass ratio of cellulose and α-amylase was 1∶1,the best technology for extracting anthocyanins from purple potato was as follows:solid to liquid ratio 1∶30(g/mL),enzyme quantity 4 mg/g,extraction time 40 min,extraction temperature 55 ℃.The ability of scavenging hydroxyl radicals of purple potato anthocyanin was investigated.The results showed that the anthocyanin had a good performance in scavenging hydroxyl radical,when the concentration was 5 mg/mL,the free radical scavenging rate was close to 100%,which was similar to that of ascorbic acid.
文章编号:202115015     中图分类号:    文献标志码:
基金项目:河南省科技厅科技攻关项目(172102310445);许昌学院科研项目(2021YB008)
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