Abstract:In this experiment，high-throughput sequencing technology was used to study the effects of black chokeberry wine on the intestinal flora of mice with acute liver injury induced by D-galactose.Thirty six Kunming mice were raised for this experiment and divided into 3 groups with equivalent average body weight:12 in the healthy control group（KB），12 in the injury model control group（MX），and 12 in the treated injury model group（HQ）.Mice were adapted to their feeding regimen for one week prior to dividing them into groups.The KB group continued to be fed normally without other treatment.The MX and HQ groups were injected with D-galactose（500 mg/kg）daily until the end of the experiment.The HQ group was administered black chokeberry wine（2 mL/kg）every day until the end of the experiment.After feeding for a total of 5 weeks，the mice were assessed for increased defecation，then sacrificed for high-throughput sequencing of the V3-V4 region of the prokaryotic 16S rRNA to explore changes in the intestinal flora of the mice.Compared with the KB group，the MX group had greater differences in flora diversity，genetic relationships，species diversity，and abundance values.The HQ group was similar to the KB group.According to the analysis of the genus level，the total biomasses comprised by the 10 most prevalent intestinal bacteria were 35.2%，24.33% and 36.36% in the KB，MX，and HQ groups，respectively.Among these，the total biomass of Bacteroides and Lactobacillus was reduced in the MX group compared with the KB group，while they were increased in the HQ group relative to the MX group，indicated that black chokeberry wine can improve and repair the intestinal flora of mice with liver injury.