Abstract:A method combining liquid chromatography-mass spectrometry with solid phase extraction was established for the determination of ftibamzone resdue in honey.The honey samples were dissolved in distilled water and purified using solid phase extraction with a phenyl column.The target compound was separated using a gradient elution process with a C18 column（3.0 mm× 100 mm，2.7μm）with two mobile phases consisting of 0.1% formic acid aqueous solution and acetonitrile.The quantitative detection was performed using an electrospray ionization source in positive ionization mode under multiple reaction monitoring mode.The standard curve was constructed and exhibited a strong linear response （R2>0.99）in the range of 5 μg/L-1 000 μg/L.The limit of detection for ftibamzone in honey was 2 μg/kg，and the limit of quantification was 5 μg/kg.The recoveries ranged from 93.67% to 99.61%，with intraday relative standard deviation of 2.74%-4.73% and inter-day relative standard deviation of 2.3%-3.87% at three spiked levels（5，50，200 μg/kg）in blank honey samples（n=6）.The method developed in this study was suitable for the rapid detection of ftibamzone residue in honey and could provide a reference for ftibamzone analysis in other foods.