Abstract:Lactic acid bacteria(LAB；12 strains)isolated from traditional Northeast sauerkraut were screened for low-temperature resistance and antagonistic activity against Listeria monocytogenes，and inhibition effects of LAB on biofilm formation by L.monocytogenes were analyzed.First，the low-temperature growth characteristics and antibacterial activity of LAB were determined.Next，the effects of LAB on the biofilm formation and morphological structure of L.monocytogenes were analyzed using quantitative and qualitative methods.Finally，the LAB strains were subjected to physiological，biochemical，and molecular(16S rDNA)characterization.The results showed that strain Z01 grew at a low temperature（10 ℃）；the value of OD595nmincreased by 0.23 after cultivation for 72 h，and it had anti-L.monocytogenes activity with the diameter of inhibition zone 19.79 mm.The minimal inhibitory concentration(MIC)of the crude extract of strain Z01 was 16.0 mg/mL.When the concentrations of the crude extract were 0.5 MIC and 1.0 MIC，the inhibitory rates for biofilm formation by L.monocytogenes were 59.33% and 77.77%，and those for biofilm adhesion were 14.49% and 26.44%，respectively.Eventually，strain Z01 was identified as Weissella confusa.Our findings would lay a foundation for the development of biological agents for controlling the growth of L.monocytogenes under low temperature conditions.