Abstract:A method of high performance liquid chromatography-tandem mass spectrometry（HPLC-MS-MS）method was developed for the simultaneous determination of epinephrine and dopamine in animal-derived foods.Animal-derived food samples were extracted with phosphate buffer and purified by weak anion exchange solid phase extraction column;the separation was performed on a Thermo Hypersil GOLD C18column（4.6mm×50mm，1.9 μm） with a mobile phase of 0.1% formic acid water and acetonitrile;HPLC-MS-MS method，positive ion mode scanning，electrospray ionization （ESI+），multiple reaction monitoring （MRM），and internal standard method were used to realize quantitative analysis.The results showed that:epinephrine and dopamine had good linearity in the range of 10 ng/mL-500 ng/mL，the correlation coefficient was greater than 0.999;the limit of quantitation in meat and meat products was 25 μg/kg，the limit of quantification of egg，milk and egg milk products was 50 μg/kg;the recoveries of epinephrine and dopamine in the range of 25 μg/kg-500 μg/kg were 81.7%-113.7%，the precision of the method was between 1.0%-10.4%（n=6）.The method can effectively reduce the matrix effect in the pretreatmentand provide a reliable method for the determination of epinephrine and dopamine in animal derived food.