Abstract:Chitin is widely used， and waste from aquatic products is often the main source of raw material for chitin preparation.In this study，shrimp shells in Huanghua were used as the source material，and chitin preparation technology was optimized using single factor and response surface experiments combining deproteinization with neutral protease，and decalcification using acetic acid.The best conditions for deproteinization were 2 h at 48 ℃with 5 000 U of neutral protease. The best conditions for decalcification were 1 h at 20 ℃，and the best technological conditions of decalcification was the ratio of water to acetic acid was 9 ∶1. Using these optimized conditions，chitin yield was 6.87%.