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投稿时间:2020-07-19
投稿时间:2020-07-19
中文摘要: 以“安海变”杨梅果实为试验材料,2 ℃~4 ℃温度条件下,常压贮藏为对照(control check,CK),研究低压[lowpressure,LP(0.05 MPa)]贮藏对杨梅果实细胞膜透性(cell membrane permeability,CMP)及其抗氧化性的影响,并监测贮藏期间细胞膜透性和抗氧化机制有关的生理生化指标动态变化。结果表明:低压贮藏诱导杨梅果实启动抗氧化保护机制,明显延缓其内源抗氧化物质抗坏血酸(ascorbic acid,AsA)高峰出现、限制其含量下降,延迟脯氨酸(proline,Pro)含量急剧上升,有效维持超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性在贮藏中期逐渐上升,并保持后期较高活性,明显抑制过氧化物酶(peroxidase,POD)和多酚氧化酶(polyphenol oxidase,PPO)活性,提高杨梅果实低温逆境抗氧化能力,减轻丙二醛(malondialdehyde,MDA)积累和膜脂过氧化程度,有效延缓CMP上升,使其在贮藏第16 天时CMP 仍保持在10%以下,减缓了杨梅果实生理衰老进程。进一步分析发现,低压贮藏诱导杨梅果实组织降低MDA 积累,延缓CMP 上升,主要是内源抗氧化物质Pro 和抗氧化相关酶POD、PPO、SOD 等关键指标发挥主导作用,而AsA、CAT 作用较不明显。
Abstract:The effects of low-pressure(LP,0.05 MPa)storage on cell membrane permeability(CMP)and anti-oxidation of'Anhaibian'bayberry fruit were studied,and atmospheric pressure storage was used as the control check(CK)during 2°C-4°C.The changes of physiological and biochemical indexes related to membrane permeability and anti-oxidation mechanisms were monitored during storage.The results showed that LP storage induced bayberry fruit to initiate the antioxidant protection mechanism,noticeably delayed the occurrence of the endogenous antioxidant ascorbic acid (AsA)peak,caused its content to decline,and delayed the sharp rise in proline(Pro)content. The activity of superoxide dismutase(SOD)and catalase(CAT)increased gradually in the middle period of storage,and retained high activity in the later period,which noticeably inhibited the activity of peroxidase(POD)and polyphenol oxidase(PPO)in bayberry fruit under low temperature stress,the malondialdehyde(MDA)accumulation and membrane lipid peroxidation decreased,CMP rise was delayed,causing CMP to remain below 10%on the 16th day of LP storage,and the physiological senescence of bayberry fruit was delayed. Further analysis showed that LP storage induced the decrease of MDA accumulation and delayed the increase of CMP in bayberry fruit,mainly due to the dominant role of Pro and POD,PPO,SOD,etc.,but the correlation with ASA and CAT was not obvious.
keywords: Chinese bayberry(Myrica rubra)fruit low pressure storage cell membrane permeability antioxidation physiological biochemistry
文章编号:202112010 中图分类号: 文献标志码:
基金项目:福建省科技创新平台建设项目(2018N2002)
作者 | 单位 |
郑俊峰,谢建华,庞杰 | 漳州职业技术学院食品与生物工程系,福建 漳州 363000;农产品深加工及安全福建省高校应用技术工程中心,福建 漳州 363000;福建农林大学食品科学学院,福建 福州 350002 |
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