本文已被：浏览 4766次 下载 629次
投稿时间：2021-01-23
投稿时间：2021-01-23
中文摘要: 以植物乳杆菌AB-1（Lactobacillus plantarum AB-1）为研究对象，探究不同培养基组成以及外源添加酵母菌无细胞发酵上清液（cell-free supernatant，CFS）对植物乳杆菌AB-1生产苯乳酸的影响。结果表明，将培养植物乳杆菌AB-1的MRS培养基中葡萄糖浓度由20.0 g/L提高至27.5 g/L、蛋白胨浓度由10.0 g/L提高至30.0 g/L时，苯乳酸产量从81.2 mg/L提高至103.8 mg/L，提高了27.8%（P<0.05）。在此基础上，外源添加15%经40 h发酵的酵母菌E1 CFS，可使苯乳酸产量达到130.2 mg/L，相较于优化MRS培养基和初始MRS培养基分别提高了25.4%和60.3%（P<0.05）。进一步研究表明，植物乳杆菌AB-1苯乳酸产量的提高可能与酵母菌CFS中苯丙氨酸（Phe）和α-酮戊二酸（α-KG）两种代谢产物有关。综上，该研究通过优化培养基及外源添加酵母菌CFS的方法，找到一种提高植物乳杆菌AB-1生产苯乳酸能力的培养方式。
Abstract:The effects on the production of phenyllactic acid（PLA）by Lactobacillus plantarum AB-1 were investigated by optimizing the medium composition and adding yeast cell-free supernatant（CFS）.The results indicated that the production of PLA by L.plantarum AB-1 increased from 81.2 mg/L to 103.8 mg/L in a modified MRS medium in which the concentration of glucose increased from 20.0 g/L to 27.5 g/L and the concentration of peptone increased from 10.0 g/L to 30.0 g/L，separately.Additionally，after adding 15%40 h-fermented yeast E1 cell-free fermentation supernatant，the production of PLA by L.plantarum AB-1 significantly increased to 130.2 mg/L，an enhancement of 60.3% and 25.4% compared with that under the original and optimal MRS culture conditions，respectively.Further investigation revealed that the increase in PLA production may be associated with the presence of phenylalanine and α-ketoglutarate in the yeast cell-free fermentation supernatant.In conclusion，this study proposes a feasible method to increase the production of PLA by L.plantarum AB-1.
keywords: Lactobacillus plantarum fermentation phenyllactic acid yeast cell-free supernatant biological preservatives
文章编号：202111024 中图分类号： 文献标志码：
基金项目:国家自然科学基金（31972141）
| Author Name | Affiliation |
| ZHANG Cheng-lin，QIAN Yi-lin，LIU Zun-ying | College of Food Science and Engineering，Ocean University of China，Qingdao 266003，Shandong，China |
引用文本：
津ICP备20000603号-1