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食品研究与开发:2021,42(10):171-178
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高耐受Pb2+酵母菌抗氧化及吸附Pb2+特性的研究
(内蒙古农业大学食品科学与工程学院,内蒙古 呼和浩特 010018)
Studies of Antioxidant and Pb2+Adsorption Characteristics of Highly Pb2+Tolerant Yeast
(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,Inner Mongolia,China)
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投稿时间:2021-01-21    
中文摘要: 对Pb2+耐受浓度在6 000 mg/L以上的异常威克汉姆酵母(Wickerhamomyces anomalus)QI-1-6、QI-1-7、QD-2-8进行体外试验。结果表明,3株酵母菌均具有一定的抗氧化能力,且菌悬液的抗氧化能力显著高于无细胞提取物,W.anomalus QI-1-6菌悬液抑制脂质过氧化能力达到(144.72±4.53)μg/mL;W.anomalus QI-1-7菌悬液对DPPH自由基、羟基自由基和超氧阴离子自由基的清除能力分别达到(85.09±1.11)、(240.88±7.69)、(171.41±6.92)μg/mL。W.anomalus QI-1-7随着pH值、吸附时间和菌体浓度增加,吸附Pb2+能力上升;Pb2+初始浓度增加,吸附率下降显著;温度增加,吸附Pb2+能力呈先上升后下降趋势。
Abstract:Wickerhamomyces anomalus strains QI-1-6,QI-1-7,and QD-2-8 which display tolerance to Pb2+concentrations above 6 000 mg/L were assessed in vitro.All three yeast strains displayed antioxidant capacity,and the antioxidant capacity of each yeast suspension was significantly higher than that of each acellular extract.W.anomalus QI-1-6 suspension yielded the highest inhibition of lipid peroxidation(144.72±4.53)μg/mL.QI-1-7 displayed the highest scavenging capacities toward DPPH and hydroxyl radicals and superoxide anions at(85.09±1.11)μg/mL,(240.88±7.69)μg/mL,and(171.41±6.92)μg/mL,respectively.With increasing pH,adsorption time,and cell concentration,the adsorption capacity of W.anomalus QI-1-7 increased;with increasing initial Pb2+concentration,the adsorption rate decreased significantly.With increasing temperature,the adsorption capacity of W.anomalus QI-1-7 initially increased,then decreased.
文章编号:202110027     中图分类号:    文献标志码:
基金项目:内蒙古自治区自然科学基金项目(2017MS0324);内蒙古自治区科技成果转化项目(CGZH2018145);内蒙古自治区研究生科研创新项目(S20191166Z)
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