Abstract:This study aimed to explore the immunomodulatory effects of polysaccharides derived from five kinds of edible fungi，including Tremella fuciformis，Poria cocos，Lentinus edodes，Hericium erinaceus and Dictyophora indusiata，as well as the compound polysaccharides obtained by mixing these polysaccharides in pairs on the mouse macrophage RAW264.7.RAW264.7 cells were divided into the normal control group，positive control group，and sample treatment（lentinan，polysaccharides derived from Tremella fuciformis，Poria cocos，Hericium erinaceus and Dictyophora indusiata，and ten complexes obtained by mixing the polysac－charides in different concentrations）groups.Macrophage viability and the levels of tumor necrosis factor（TNF）-α were evaluated to examine the immunoregulatory effect as well as the phagocytic ability of RAW264.7 cells following treatment.Compared with the normal control group，polysaccharides derived from the five edible fungi significantly enhanced cell viability，promoted the secretion of the cytokine TNF-α，and improved the phagocytic ability of RAW264.7 cells （P<0.05）with concentrations ranging from 5 μg/mL to 320 μg/mL.In addition，the ten polysaccharide complexes demonstrated significant ability to promote cell viability，TNF-α secretion，and phagocytosis of RAW264.7 cells.The compound polysaccharide composed of lentinan and the polysaccharide derived from Hericium erinaceus were most effective in promoting the secretion of TNF-α at a concentration of 160 μg/mL.The compound polysaccharide composed of lentinan and the polysaccharide derived from Poria cocos demonstrated the strongest enhancement of the phagocytic function of cells；this effect was superior to those of its two components.The compound polysaccharide composed of the polysaccharides derived from Tremella fuciformis and Dictyophora indusiata promoted the secretion of TNF-α by RAW264.7 cells.This effect was stronger than those of its component polysaccharides at a concentration of 80 μg/mL.The ability of the complex to enhance the phagocytic function of cells was stronger than those of its component polysaccharides at a concentration of 160 μg/mL.Polysaccharides derived from Tremella fuciformis，Poria cocos，Lentinus edodes，Hericium erinaceus，and Dictyophora indusiata and their complexes regulated the immune response of the RAW264.7 macrophage line.Different polysaccharides might interact，and the immunoregulatory activities of the compound polysaccharides were superior to those of their component polysaccharides，including lentinan and the polysaccharides derived from Poria cocos，Tremella fuciformis and Dictyophora indusiate.