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投稿时间：2020-07-24
投稿时间：2020-07-24
中文摘要: 以圆铃1号枣为试验材料，通过单因素和正交试验优化其多酚的提取条件，并对其酚酸组成、体外抗氧化能力进行初步研究。结果表明：乙醇体积分数为60%、提取温度为60℃、料液比1∶16（g/mL）、提取时间20 min时，多酚提取量最大，达到（10.69±0.078）mg/g。采用高效液相色谱法鉴定出6种酚酸，分别为芦丁、香豆素、咖啡酸、肉桂酸、（+）-儿茶素、绿原酸，其中芦丁的含量最高，为（17.09±0.48）mg/100 g DW。其多酚提取物的DPPH·清除能力为（17.96±0.95）mg Trolox/g、ABTS+·清除能力为（27.79 ± 3.06）mg Trolox/g、亚铁离子还原力为（32.89 ± 2.17）mg Trolox/g，是一种极具潜力的天然抗氧化剂。
Abstract:The extraction conditions of polyphenols from‘Yuanling 1’jujube was optimized by single factor and orthogonal tests，and the phenolic acid composition and antioxidant capacity of the polyphenols were preliminarily studied.The results showed that the extraction of polyphenols reached（10.69 ± 0.078）mg/g when the volume fraction of ethanol was 60%，the extraction temperature was 60 ℃，the solid-liquid ratio was 1∶16（g/mL）and the extraction time was 20 min.Six phenolic acids were identified by high performance liquid chromatography（HPLC）：rutin，coumarin，caffeic acid，cinnamic acid，（+）-catechin and chlorogenic acid，among which rutin content was the highest（17.09 ± 0.48）mg/100 g DW.The DPPH·scavenging capacity of polyphenol extract was （17.96 ± 0.95）mg Trolox/g，ABTS+·scavenging capacity was （27.79 ± 3.06）mg Trolox/g，and the ferric ion reducing capacity（FRAP）was（32.89 ± 2.17）mg Trolox/g.It is a natural antioxidant with great potential.
文章编号：202108009 中图分类号： 文献标志码：
基金项目:山东省重点研发计划项目（2016GNC113015）；山东省2017年度农业重大应用技术创新项目（SDNY2017006）；2019年度山东省重点研发计划（公益类专项）（2019GNC106061）；佳县红润枣业专业合作社横向课题（SDAUHX2018009）
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