Abstract:The anti fatigue effects of flavonoids from Moringa oleifera leaves on overtraining in mice was studied.The mice were randomly divided into five experimental groups （50 mice，10 in each group），which were flavonoids from Moringa oleifera leaves low-dose group[100 mg/（kg·d）]，medium-dose group[200 mg/（kg·d）]，high-dose group[400 mg/（kg·d）]，model group[distilled water 100 mg/（kg·d）]and control group[rhodiola and American ginseng capsule 200 mg/（kg·d）].The mice were gavaged and overtraining for 8 weeks，then the low-dose，medium-dose and high-dose groups were compared with the model group and control group to evaluate the levels of anti-fatigue.The results indicated that，compared with the model group，the reserve of hepatic glycogen and muscle glycogen in the low-dose，medium-dose and high-dose groups significantly increased and the activity of catalase，superoxide dismutase and glutathione peroxidase significantly increased in serum and liver tissue （P＜0.05）.In addition，the levels of lactic acid，urea nitrogen and malondialdehyde significantly decreased and the activity of lactate dehydrogenase and creatine kinase significantly decreased in serum（P＜0.05）.Furthermore，compared with the control group，all differences were not significant in the medium-dose and high-dose group.Flavonoids from Moringa oleifera leaves can relieve sports fatigue.