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投稿时间：2020-06-03
投稿时间：2020-06-03
中文摘要: 该文主要以始兴黄精为原料，纯净水为提取溶剂，采用超声波辅助酶法提取黄精多糖，通过单因素试验研究复合酶添加量、酶解时间、酶解温度和料液比等因素对黄精多糖提取率的影响，并对其最佳工艺进行正交试验优化。结果表明，超声波辅助酶法提取黄精多糖的最佳工艺条件为：复合酶添加量6%、酶解温度65 ℃、酶解时间55 min、料液比1 ∶30（g/mL），在此工艺条件下得到黄精多糖的提取率为25.63%。
Abstract:To used Shixing Polygonatum as the raw material，purified water as the extraction solvent，and used ultrasonic-assisted enzymatic extraction of Polygonatum polysaccharides. Through single factor experiments，the effects of compound enzyme addition，digestion time，digestion temperature and material-liquid ratio on the extraction rate of Polygonatum polysaccharides were studied，and optimize the best process by orthogonal experiments.The results showed that the optimal process conditions of ultrasonic-assisted enzymatic extraction of Polygonatum polysaccharides were 6%compound enzyme addition，enzymolysis temperature 65 ℃，enzymolysis at a time of 55 min and a material-to-liquid ratio of 1 ∶30（g/mL），the extraction rate of Polygonatum polysaccharide under this process condition was 25.63%.
keywords: ultrasonic assisted enzymatic method Polygonatum Polygonatum polysaccharides process optimization extraction rate
文章编号：202107022 中图分类号： 文献标志码：
基金项目:韶关市科技计划项目（2018sn046、2018sn155、2008111545 32467）；韶关学院第二十批（2019 年度）校级教育教学改革项目（SYJY20192019）；韶关学院校级课题（SY2016KJ04）；2020 年韶关学院大学生创新创业训练计划项目（S202010576030）
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