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食品研究与开发:2021,42(7):83-89
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超高温巴氏杀菌对全蛋液功能性质和理化性质的影响
(吉林农业大学食品科学与工程学院,吉林长春130118)
Effects of Ultra-Pasteurization on the Physicochemical and Functional Properties of Liquid Whole Egg
(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,Jilin,China)
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投稿时间:2020-04-05    
中文摘要: 该文首先比较超高温巴氏杀菌、传统巴氏杀菌对全蛋液中3 种不同病原微生物的灭活效果,然后通过分析全蛋液起泡性、泡沫稳定性、乳化性、乳化稳定性、溶解度、巯基含量、表面疏水性质、十二烷基硫酸钠聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE),比较超高温巴氏杀菌、传统巴氏杀菌全蛋液在冷藏(4 ℃)过程中功能性质和理化性质的变化趋势。结果表明,超高温巴氏杀菌相对于传统巴氏杀菌更为有效地灭活全蛋液中的大肠杆菌、沙门氏菌、金黄色葡萄球菌。超高温巴氏杀菌与传统巴氏杀菌全蛋液的起泡性、泡沫稳定性均显著高于未杀菌,但二者之间没有显著差异,冷藏1 周后,三者起泡性均显著下降,而泡沫稳定性呈上升趋势,但是随着冷藏时间延长至6 周,三者均未呈现显著变化。超高温巴氏杀菌全蛋液乳化性及乳化稳定性为先增加后降低,在整个储藏过程中,两者间均无显著性差异。不同杀菌方式对全蛋液中蛋白质溶解度、游离巯基影响不显著。超高温巴氏杀菌对全蛋液功能性质和理化特性影响与传统巴氏杀菌没有显著差异,为其在全蛋液工业生产的应用提供理论依据。
Abstract:Effects of ultra -pasteurization and traditional pasteurization on three different pathogenic microorganisms in liquid whole egg were compared. Addititonally,the corresponding changes in the functional and physicochemical properties of samples upon pasteurization were investigated under cold-storage conditions(4 ℃). Toward this end,the foaming,foaming stability,emulsification,emulsification stability,solubility,sulfydryl content,hydrophobic properties,and whole -protein profile (via sodium dodecyl sulfate -polyacrylamide gel electrophoresis)of the samples were evaluated.The results showed that ultra-pasteurization was more effective than the traditional pasteurization in reducing the numbers of Escherichia coli,Salmonella typhimurium,and Staphylococcus aureus in liquid whole egg. The samples pasteurized either way had significantly more foaming and foaming stability than the unsterilized egg but did not significantly differ from each other. After refrigeration for 1 week,the foaming in all the samples significantly decreased. However,foaming showed an upward trend;but with the extension of the refrigeration period to 6 weeks,none of the samples showed significant changes. There was no significant difference in emulsification or emulsification stability among the samples. The difference in sterilization caused no significant difference in the resulting protein solubility or free sulfydryl content. In conclusion,the effects of ultra-pasteurization on the functional and physicochemical properties of liquid whole egg are not significantly different from those of the traditional pasteurization. These observations provided a theoretical basis for the application of ultra-pasteurization to liquid whole egg in industrial settings.
文章编号:202107014     中图分类号:    文献标志码:
基金项目:十三五国家重点研发计划(2018YFD0400304)
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