Abstract:The effects of the ultrasonic power，extraction temperature，complex enzyme dosage，solid-toliquid ratio，and extraction time on the yield of polysaccharides extracted from jujube were investigated by single factor experiments.The response surface methodology coupled with a genetic algorithm was used to optimize the ultrasonic-assisted complex enzyme extraction （UACEE）process of jujube polysaccharides；moreover，the effects of different extraction methods on the yield of polysaccharides from jujube was explored via comparative methods.The results showed that the optimum extraction parameters to achieve the highest polysaccharide yield （7.68±0.01）% from jujube by UACEE were as follows：ultrasonic power of 308 W，extraction temperature of 40℃，complex enzyme dosage of 0.49%，solid-to-liquid ratio of 1∶32（g/mL），and extraction time of 30 min.The relative error between the experimental and theoretical values was 3.09%.The results showed that the response surface method coupled with the genetic algorithm could simulate and predict the polysaccharide yield from jujube；and the optimization of the process parameters was feasible.