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投稿时间：2020-11-03
投稿时间：2020-11-03
中文摘要: 以羧基化的Fe3O4 纳米粒子为载体，6-苄氨基腺嘌呤为模板分子，甲基丙烯酸和对苯乙烯磺酸钠为复合功能单体，采用表面印迹技术制备磁性分子印迹纳米粒子，优化其制备条件和富集分离6-苄氨基腺嘌呤的条件。结果表明，当甲基丙烯酸和对苯乙烯磺酸钠的物质的量比为3∶1，羧基化Fe3O4 纳米粒子的添加量为0.25 g，洗脱液甲醇-乙酸溶液的体积比为9∶1 时，所制备的磁性分子印迹纳米粒子吸附性能最佳，其吸附容量和特异性均优于相同条件下基于单功能单体制备的磁性分子印迹纳米粒子。应用所制备的磁性分子印迹纳米粒子12 mg，对4 mL 20 μg/mL 的6-苄氨基腺嘌呤进行静置吸附，其吸附效率可达94.10%。该研究为蔬菜中残留的6-苄氨基腺嘌呤的高效富集分离，提供了一种简便快速的方法。
Abstract:Magnetic molecular imprints nanoparticles（MMIPs NPs）were synthesized using Fe3O4 nanoparticles（Fe3O4 NPs）as carrier material，6-benzylaminopurine（6-BA）as template molecule，methacrylic acid（MAA）and sodium 4-vinylbenzenesulfonate （SSS）as combined functional monomers based on the surface molecular imprinting technology. The conditions for the MMIPs NPs preparation and enrichment and separation of 6-BA using MMIPs NPs were optimized.When the amount ratio of MAA and SSS was 3∶1，the amount of carboxylated Fe3O4 NPs added was 0.25 g，and the volume ratio of the methanol and acetic acid solution was 9∶1，the MMIPs NPs with best adsorption performance were successfully prepared.The adsorption capacity and specificity of the prepared MMIPs NPs were better than those of the prepared MMIPs NPs using monofunctional monomers under the same conditions. The adsorption efficiency could reach 94.10% when the concentration of 6-BA solution was 20 μg/mL（4 mL），the addition of MMIPs NPs was 12 mg.This study provided a simple and rapid method for the efficient enrichment and separation of 6-BA residues from fruits and vegetables.
keywords: magnetic nanoparticles bi-functional monomer molecularly imprinted polymers enrichment and separation 6-benzylaminopurine
文章编号：202105015 中图分类号： 文献标志码：
基金项目:国家自然科学基金（31671931）；湖南省自然科学基金（2020JJ4354）
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