Abstract:To explore the effects on the inactivation of different types of food-borne spoilage microorganisms，E.coli and S.aureus were treated by atmospheric cold plasma（ACP）.The effects of ACP treatment time，power and electrode spacing on colony number were investigated.The results showed that the more reduction of E.coli and S.aureus colony was observed with the increasing treatment time，increasing power or decreasing electrode spacing.Moreover，the changes in morphology，protein leakage and malondialdehyde production of E.coli and S.aureus via ACP were measured，indicating that the inactivation of E. coli and S.aureus was induced by the rupture of the oxidation-induced extracellular membrane and by the etching of the extracellular membrane caused by the physical impact of charged particles.