Abstract:To investigate the effect of the anti fatigue of Ganoderma atrum polysaccharides on mice after weight-loading swimming.The mice were randomly divided into 5 experimental groups（50 mice，10 in each group），including low-dose group[25 mg/（kg·d）]，medium dose group[50 mg/（kg·d）]，high-dose group[100 mg/（kg·d）]，blank group（distilled water）and positive control group[rhodiola and American ginseng capsule 200 mg/（kg·d）].After 30 days of gavage，Ganoderma atrum polysaccharides low，medium and high dose groups were compared with the blank group and the positive control group to evaluate the anti fatigue ability of the mice.Compared with the blank group，the weight-loading exhaustive swimming time of mice in the low，medium and high dose groups of Ganoderma atrum polysaccharide significantly increased（P＜0.05），the contents of blood glucose，liver glycogen and muscle glycogen were significantly increased （P＜0.05），the contents of lactic acid，urea nitrogen，creatine kinase and lactate dehydrogenase in serum were significantly decreased（P＜0.05），in serum and liver tissue the malondialdehyde content was significantly decreased（P＜0.05）and the activities of superoxide dismutase，catalase and glutathione peroxidase were significantly increased（P＜0.05），the activities of Na+K+-adenosine triphosphate（ATP）ase and Ca2+Mg2+-ATPase in liver tissue were significantly increased（P＜0.05）.Compared with the positive control group，there was no significant difference between the medium and high dose groups.Ganoderma atrum polysaccharides can effectively ameliorate exercise-induced fatigue.