Abstract:Fresh-cut pineapples are easy to be contaminated by spoilage bacteria，leaing to the decline in fruit quality and the reduction in shelf life.To reduce the initial colony number of fresh-cut pineapples without causing quality change，plasma-activated water（PAW）was generated by atmospheric pressure cold plasma（ACP）treatment，and its germicidal efficacy for fresh-cut pineapples were investigated.The effects of PAW preparation time，PAW preparation power and PAW washing time on colony number were researched.The optimal sterilization conditions by single factor and orthogonal test showed that PAW preparation time of 4 min，PAW preparation power of 70 W，PAW washing time of 20 min，the colony number of fresh-cut pineapples dropped from 3.035 lg （CFU/g）to 2.604 lg （CFU/g）.The chrominance，firmness，sensory quality，pH value and vitamin C content of fresh-cut pineapple were measured，the results showed that PAW could effectively reduce the initial colony number of fresh-cut pineapples while maintaining its original quality.The shelf life of fresh-cut pineapples was predicted by acceleration test，results indicated that，at storage temperature of 4 ℃，the shelf life of fresh-cut pineapple treated by PAW was extended from 8.06 h to 9.03 h.