Abstract:The microwave-assisted extraction and response surface methodology were used to optimize the extraction parameters of flavonoids from the fruits of Ribes stenocarpum Maxim.in Qinghai-Tibet plateau.The inhibitory rate of pancreatic lipase and the binding ability of different cholic acid salts were used to evaluate the hypolipidemic activity in vitro of Ribes stenocarpum Maxim.before and after purification.The results showed that the optimum conditions of microwave-assisted extraction were as follows：microwave power 500 W，material-liquid ratio 1∶30 （g/mL），extraction time 8 min，the extraction rate was 22.36 %.The hypolipidemic activity in vitro after purification was relatively strong.The inhibition rate of pancreatic lipase was 91.6 %，it was 16.81 % higher than before purification；and the IC50of sodium cholate，sodium taurocholate and sodium glycocholate were 3.301，1.499，1.847 mg/mL respectively.Indicating that Ribes stenocarpum Maxim.had the prospect of developing natural functional foods for hypolipidemic，and flavonoid was the main active ingredient in the fruits of Ribes stenocarpum Maxim..