Abstract:The crude flavonoids from eight edible fungi were extracted using simulated digestion method and ultrasonic alcohol extraction method，and thereafter the contents and activities of reduction and hydroxyl free radical elimination of crude flavonoids extracted from these two methods were compared.The results showed that the contents of crude flavonoids in eight edible fungi extracted from simulated digestion method ranged from 1.25 mg/g to 7.85 mg/g，which was 20.3%-509.6% higher than those extracted from ultrasonic alcohol extraction method.Additionally，the EC90 of hydroxyl radical scavenging abilities（concentration for 90% of maximal effect）and the EC50 of reduction activities of crude flavonoids extracted from simulated digestion method were 33.64%-3 216.67% and 19.87%-151.76% higher than those from ultrasonic alcohol extraction method，respectively.Therefore，utilizing simulated digestion method to treat fruiting body of edible fungi could promote the release of flavonoids and enhance their antioxidant activity，which could provide the theoretical reference for further development of the functional activity of edible fungi.