Abstract:Apple fruit （cv.Golden Delicious）was used as the materials to study the effect of acibenzolar-S-methyl（ASM）dipping treatment for 10 min on the key enzymes activities and antifungal compounds in phenylpropanoid pathway during room temperature storage.The fruit treated with distilled water was used as the control.The results demonstrated that ASM treatment significantly enhanced the activity of phenylalamine ammonia lyase，polyphenol oxidase，4-coumarate coenzyme A ligase and cinnamate 4-hydroxylase in apple fruit.The accumulation of total phenolic compounds and flavonoids were also increased in the ASM-treated apple fruit.These results suggested that postharvest ASM treatment enhanced the accumulation of phenolic compounds and flavonoids by triggering phenylpropanoid pathway，therefore reinforcement the cell wall in apple fruit.