Abstract:In the present study，oolong tea Tieguanyin stalks were used as raw materials，to optimize the compound enzyme extraction technology of tea polyphenols from the oolong tea stalks by Box-Behnken response surface methodology.The single-factor experiment and response surface methodology were adopted to study the effects of compound enzyme addition，ratio of solid to liquid and enzymatic hydrolysis temperature on the extraction yield of tea polyphenols from oolong tea stalks.The results showed that the best technology parameter combination of the extraction of tea polyphenols from tea stalks were as follows：enzyme addition amount was 1.2%（based on substrate weight），ratio of solid to liquid was 1 ∶20（g/mL）and enzymatic hydrolysis temperature was 48℃.Under these conditions，tea polyphenols extraction content was 82.26 mg/g，which reached the predicted optimal value.