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投稿时间：2020-02-04
投稿时间：2020-02-04
中文摘要: 采用顶空固相微萃取（headspace solid-phase micro extraction，HS-SPME）对9种不同组成的培养基栽培的香菇挥发性成分进行提取，用气相色谱-质谱联用（gas chromatography-mass spectrometry，GC-MS）技术进行鉴定，并通过软独立模式分类（soft independent modeling of class analogy，SIMCA）进行主成分分析。结果表明：9种培养基生产的香菇挥发性成分的种类差异不大，分布在36种～43种之间，主要是烃类、醇类、酮类物质。主成分分析发现，柞木生产的香菇和果木生产的香菇挥发性成分相对较大。前两个主成分反应了原始变量72.1%的信息，对第一主成分贡献最大的是3-辛醇，其次是3-甲基-1-丁醇、3-辛酮、2-甲基-1-丁醇、2-乙基己醇；对第二主成分贡献最大的是二甲基四硫，其次是1，2，4-三硫戊烷、二甲基三硫、二甲基二硫。
Abstract:The volatile components of Lentinula edodes cultivated in 9 different media were extracted by headspace solid-phase micro extraction（HS-SPME）and identified by gas chromatography-mass spectrometry（GC-MS）.The principal components were analyzed by soft independent modeling of class analogy（SIMCA）software.The results showed that there was no significant difference among 9 kinds of volatile components，the number of volatile components identified was from 36 to 43，mainly including hydrocarbons，alcohols and ketones.The results of principal component analysis showed that the volatile components of Lentinula edodes produced by tussah and fruit wood were relatively large.The first two principal components represented 72.1%of the total variability，and 3-octanol，3-methyl-1-butanol，3-Octanone，2-methyl-1-butanol and 2-ethylhexanol were biggest contributor to the first principal component.Dimethyl tetrasulfide，1，2，4-trithiolane，dimethyltrisulfide and methyl disulfide were biggest contributor to the second principal component.
keywords: Lentinula edodes aroma components culture medium gas chromatography-mass spectrometry principal components analysis
文章编号：202024004 中图分类号： 文献标志码：
基金项目:河北省重点研发计划（18227146）；河北省教育厅青年基金项目（QN2017351）；廊坊市科技计划项目（2019012001）；河北省大学生创新创业训练计划（201910100055）
| 作者 | 单位 |
| 梁怡，刘改霞，李朋泰，郭红珍，褚卓栋，周志国，尚校兰 | 廊坊师范学院生命科学学院，河北廊坊065000；河北省食药用菌资源高值利用技术创新中心，河北廊坊065000；河北省高校食药用菌资源开发应用技术研发中心，河北廊坊065000；巴彦淖尔市食品药品检验所，内蒙古巴彦淖尔015000 |
| Author Name | Affiliation |
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