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投稿时间：2020-01-05
投稿时间：2020-01-05
中文摘要: 为研究黄果茄总黄酮提取工艺和体外抗氧化活性，采用超声波辅助提取黄果茄总黄酮，在单因素试验的基础上，设计L9（34）正交试验优化黄果茄总黄酮的提取工艺，通过显色反应对总黄酮的结构类型进行初步推测，并对总黄酮进行体外抗氧化活性研究。结果显示，黄果茄总黄酮最佳提取工艺条件为：提取时间40 min，料液比1∶70（g/mL），温度50℃，乙醇体积分数50%，在此条件下黄果茄总黄酮提取率达4.35%；显色反应表明，黄果茄总黄酮的结构类型可能有二氢黄酮类、黄酮醇类、黄酮类和异黄酮类；体外抗氧化活性试验表明，黄果茄总黄酮具有较强的清除·OH和DPPH·能力。
Abstract:To research extraction process and antioxidant activity of total flavonoids from Solanum xanthocarpum.On the basis of single-factor test，total flavonoids from Solanum xanthocarpum were extracted by ultrasonic assisted method，the extraction process of total flavonoids was optimized by L9（34）orthogonal test，the structure type of total flavonoids was preliminarily predicted by color reaction，and the antioxidant activity in vitro of extracts was studied.The optimal extraction conditions were time of 40 min，solid-liquid ratio of 1∶70（g/mL），temperature of 50℃，and alcohol concentration of 50%.Finally，the extraction rate of total flavonoids from Solanum xanthocarpum under the optimal condition was up to 4.35%.The structural types of total flavonoids of Solanum xanthocarpum might include flavones，dihydroflavones，chalcone and isoflavone.And the extracts of Solanum xanthocarpum could effectively scavenge hydroxyl radicals and DPPH radicals.
keywords: Solanum xanthocarpum total flavonoid ultrasonic assisted extraction orthogonal test in vitro antioxidant activity
文章编号：202023024 中图分类号： 文献标志码：
基金项目:普洱学院创新团队项目（CXTD014）；普洱学院科研项目（2018xjkt03）
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