Abstract:In order to make full use of lonicera edulis and increase their economic value，the enzymatic hydrolysis method was studied to improve the juice yield of lonicera edulis and the changes in the content of four active substances in lonicera edulis juice were studied，and the scavenging rates of DPPH·，·OH，ABTS+free radicals were used to evaluate the antioxidant capacity of lonicera edulis juice.The response surface method was used to optimize the enzymatic hydrolysis process of lonicera edulis.The results showed that the optimal enzymatic hydrolysis process was 0.27% pectinase.The cellulose addition amount was 0.92%，and the hydrolysis temperature was 47℃.Under these process conditions，the juice yields of lonicera edulis was 85.67%.After enzymatic hydrolysis，the contents of VC，anthocyanins and total flavonoids in the lonicera edulis juice increased by 1.78，1.13 mg/mL and 2.54 mg/mL.Enzymatic hydrolysis had a positive effect on VC，anthocyanins，total phenols and total flavonoids in lonicera edulis juice.The IC50values of the enzymatically hydrolyzed lonicera edulis juice against DPPH·，·OH and ABTS+free radicals respective were 0.285，6.37 mg/mL and 11.62 mg/mL，and had high scavenging ability.When the mass concentration was 1.8 mg/mL，the clearance rate of DPPH free radicals by lonicera edulis juice was 88.13%.When the mass concentration was 12 mg/mL，the scavenging rate of lonicera edulis juice to·OH radicals was 74.12%.When the mass concentration of lonicera edulis juice was 60 mg/mL，the clearance of ABTS+·reached a maximum of 94.67%.