Abstract:Albumin from tartary buckwheat was extracted by Osborne classification method.Select and extract the albumin ratio，mixing time，and mixing temperature for extracting albumin as a single factor，and the extraction rate of albumin was taken as the index.On the basis of single factor research，a three-factor and three-level design method was adopted to optimize the extraction process of water-soluble albumin of bitter buckwheat by response surface method.The optimal extraction process of water-soluble albumin of bitter tartary buckwheat was determined as follows：ratio of material-to-liquid：1∶19.48（g/mL），the stirring time was 59.84 min，and the stirring temperature was 35.05℃.Considering the convenience of actual operation，the above conditions are corrected to the material-to-liquid ratio 1∶20（g/mL），the stirring time 60 min，and the stirring temperature 35℃.Under these conditions，the protein extraction rate was 35.86%，which was close to the predicted value of 36.16%.