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投稿时间：2019-12-26
投稿时间：2019-12-26
中文摘要: 为筛选出适宜分离黄芪总皂苷的大孔吸附树脂，并优选出适宜的富集工艺，通过静态试验，确定大孔吸附树脂的选型，通过建立吸附动力学模型，确定吸附时间、提取液pH值、吸附温度；通过动态试验，确定最佳装载量、洗脱液浓度和流速。结果表明：XDA-4型大孔吸附树脂对黄芪中黄芪总皂苷的吸附和分离性能最强，其最佳工艺为黄芪提取液中的黄芪总皂苷的初始浓度控制在0.72 mg/mL，pH自然，吸附温度20℃，吸附时间240 min，上样体积为80 mL，80%乙醇以1.5 BV/h的速度进行洗脱。XDA-4对黄芪总皂苷的分离纯化效果最佳，优化后工艺条件稳定，适用于黄芪中黄芪总皂苷的富集。
Abstract:To screen out a macroporous adsorption resin suitable for the separation of astragalus saponin was selected and a suitable enrichment process was optimized.Through static experiments，the selection of macroporous resin was determined.The adsorption kinetic model was used to determine the adsorption time，the pH of the extraction solution，the adsorption temperature.Through dynamic experiments，the optimal loading capacity，eluent concentration，and flow rate were determined.XDA-4 macroporous adsorption resin had the strongest adsorption and separation properties of astragalus saponins.The best process was：the initial concentration of astragalus saponins was controlled at 0.72 mg/mL，the pH was natural，and the adsorption temperature was 20℃，the adsorption time was 240 min，the loading volume was 80 mL，and 80% ethanol was eluted at a rate of 1.5 BV/h.XDA-4 had the best effect on the separation and purification of astragalus saponins.
keywords: Astragalus membranaceus astragalus saponins static adsorption and dissociation dynamic adsorption and elution
文章编号：202023016 中图分类号： 文献标志码：
基金项目:山西省应用基础研究计划项目（201801D221240）；大同市科技计划项目（2017015）
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