Abstract:The study was designed to determine the effect of compound ginseng-antler liquor and single herbs liquor in Mice.The mice were randomly divided into 9 groups：blank control group，wine group.Low，medium and high three dose groups of compound ginseng-antler were set，ginseng group，antler group，Polygonatum sibiricum group，rhodiola group.All groups were treated by gavage for 30 consecutive days，Then the Antifatigue effect was analyzed by fatigue-related iochemical indicators，including the loaded-swimming time，blood urea nitrogen，blood lactic acid and liver glycogen.The results showed that the compound（M）group，compound（H）group and all single herbs groups could increase the swimming endurance of mice（P<0.05）.The compound（M）group，ginseng group and Rhodiola group could reduce the content of urea nitrogen in mice（P<0.05）.The area under the curve of serum urea of compound （M）group，antler group and Rhodiola group in exercise mice was significantly decreased（P<0.05）.Ginseng，antler，polygonatum sibiricum and Rhodiola had synergistic effect of relieving physical fatigue.The Chinese traditional medicines in formula had a good synergy effect of relieving physical fatigue.