Abstract:To investigate Pu′er tea′s improvement of insulin resistance on SD rats fed with high-fat diet，fifty SD rats were randomly and on average divided into five groups including the normal and high fat control groups，and three Pu'er tea groups with low（0.45 g/kg·bw），middle（0.90 g/kg·bw），and high（1.35 g/kg·bw）intakes，respectively.The body weight of rats，the levels of fasting blood glucose（FBG），insulin（INS），and hepatic glycogen were determined.Furthermore，the mRNA expression level of glucose kinase（GK），glycogen synthetase（Gys-2），glucose-6-phosphatase（G6pc），and insulin receptor（INSR）in the liver，glucose transporters-4（GLUT-4）and peroxisome proliferators activated receptors-γ（PPAR-γ）in epididymal adipose tissue were also measured via using real-time polymerase chain reaction（PCR）technique.The result demonstrated that administration of Pu′er tea caused the decreases in body weight，fat index，FBG，INS levels and the ex－pression level of G6pc，and the increases in the expression level of GK，Gys-2 and INSR.Meanwhile，Pu'er teacould markedly elevatethe mRNA expression level of GLUT-4 and PPAR-γ，indicating that Pu'er tea could improve insulin resistance，reduce the blood glucose level and prevent people from diseases related insulin resistance and type II diabetes due to increasing the gene expression levels of GK，Gys-2，INSR，PPAR-γ and GLUT-4，and decreasing G6pc level.