Abstract:The dry matter of Grifola frondosa was used as raw material，optimization of complex enzymes synergistic microwave-assisted extraction of the polysaccharide by the Box-Behnken method of response surface.The sevage method was used to deproteinize，001×7 macroporous resins were used to decolorize and the components separated by DEAE-52 cellulose chromatography column were analyzed antioxidant.The test results of response surface showed that the optimal extraction conditions for Grifola frondosa polysaccharides were as follows：under the conditions of microwave time of 7 min，solid-water ratio of 1∶27 （g/mL），complex enzymes ratio of 1 ∶1 （mass ratio），and enzyme-solid ratio of 0.02 g/g，The extraction of Grifola frondosa polysaccharide was 73.25 mg/g，and the relative error with the expected value was 3.17%.Two kind of polysaccharides GFP1 and GFP2 were eluted by pure water and 0.05 mol/L NaCl on DEAE-52 cellulose chromatography column.The in vitro antioxidant test results showed that the reducing power reached 0.43 and 0.48 when the concentrations of the two polysaccharides were 6 mg/mL.The highest DPPH radical scavenging were 33.54%and 50.38%.The highest hydroxyl radical scavenging were 81.59%and 90.21%.The superoxide anion scavenging rate were 79.23%and 87.23%.The GFP2 had better antioxidant activity than GFP1.