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投稿时间：2020-07-02
投稿时间：2020-07-02
中文摘要: 以大果青扦叶为材料，采用超声辅助提取总黄酮，在单因素试验的基础上，采用Box-Behnken设计对大果青扦叶总黄酮的超声辅助提取工艺优化，并研究其抗氧化活性。结果表明最佳提取工艺为：液料比40∶1（mL/g），提取次数2次，超声时间63 min，超声功率480 W，提取温度52℃，乙醇浓度50%，总黄酮提取率为12.12%。在体外大果青扦叶总黄酮对DPPH自由基、ABTS+自由基和亚硝酸盐表现出较好的清除效果，半抑制浓度IC50值分别为1.05、0.35、1.98 mg/mL，表明大果青扦叶总黄酮具有较强的抗氧化能力。
Abstract:The extraction of total favonoids from the leaves of Picea neoveitchii was carried out by ultrasonic assisted extraction （UAE）.On the basis of single-factor experiment，the response surface method was used to optimize the ultrasonic extraction process of total flavonoids from Picea neoveitchii leaves，the scavenging rates of DPPH，ABTS+and nitrite radicals were used to study the antioxidant activity of total flavonoids.The results showed that the optimal extraction process was as follows：the liquid-to-material ratio was 40 ∶1（mL/g），the number of extractions was 2 times，ultrasonic time was 63 min，ultrasonic power was 480 W，extractive temperature was 52℃，the ethanol concentration was 50%.Under the above conditions，the extraction yield of total flavonoids was up to 12.12%.The half inhibitory concentration（IC50）for scavenging of DPPH free radical，ABTS+free radical and nitrite were 1.05，0.35，1.98 mg/mL，respectively.The result indicated that the antioxidant activity of total flavonoids from P.neoveitchii leaves was strong in vitro.
keywords: Picea neoveitchii flavonoids ultrasonic assisted extraction response surface methodology antioxidant activity
文章编号：202022006 中图分类号： 文献标志码：
基金项目:国家自然科学基金（31700597）；河南省高等学校重点科研项目（16A210035）；平顶山学院青年科研基金（PXYQNJJ2016018）；平顶山学院高层次人才科研启动基金（PXY-BSQD2016005）；平顶山学院国家级科研项目培育基金（PXY-PYJJ2017005）
| 作者 | 单位 |
| 李艳艳，王俊青，刘王文，陈壮，王晓欢 | 平顶山学院，河南平顶山467000；平顶山市药用植物功能成分利用工程技术研究中心，河南平顶山467000；河南省伏牛山区濒危植物繁育工程技术研究中心，河南平顶山467000 |
| Author Name | Affiliation |
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