Abstract:To explore the safety of Metschnikovia pulcherrima T-2 as a postharvest fresh-keeping preparation for animals and humans beings，the acute toxicity was determined by the limited method，and the toxic effect of Metschnikowia pulcherrima T-2 on mice was systematically evaluated by clinical observation，hematological analysis，blood biochemical detection，organ coefficient and pathological examination.The results showed that the acute oral toxicity of the strain T-2 fermentation broth was “non-toxic”（LD50＞10 g/kg）；mice from different dose group did not appear toxic symptoms，even these mice had normal locomotoractivity，feeding and water intake and no dead individuals.During the test，the body weight，organ coefficient，hematology index，blood biochemical index，and histopathological changes of the mice in each dose group were not significantly different from the control group （P＞0.05）.In conclusion，Metschnikowia pulcherrima T-2 was non-toxic and safe for animal organisms and had the value of further research and development as a postharvest fresh-keeping preparation.