Abstract:The aim of this study was to improve the detection efficiency of Salmonella Ⅲ b in chocolate，3 different chocolate samples were detected on the basis of the method described in“National Food Safety Standard Food microbiological examination Detection of Salmonella”（GB 4789.4-2016）.Meanwhile，BAX Q7 system，VITEK2 system and RiboPrinter system were applied to corroborate the results of the traditional method.Different enrichment and isolation conditions were analyzed.The results showed that only the sample 0716 was detected Salmonella Ⅲ b 60：r：e，n，x，z15，and polluted by different other bacterium.Selenite cystine（SC）was better applied to detect salmonella in the samples polluted by simple microflora.Tetrathionate broth（TTB）and rappaport-vassiliadis（RV）were better applied in the samples polluted by complicated microflora，and TTB was a better choice than RV when detected Salmonella Ⅲ b.Enrichment broth SC was better applied to detect salmonella in chocolate by BAX Q7 system.VITEK2 system and RiboPrinter system were able to identify salmonella at species levels，and the latter could identify serotype of Salmonella Ⅲ b.In conclusion，these detection methods could validate each other.And enriched by TTB，isolated by xylose lysine desoxycholate（XLD）or Salmonella chromogenic medium，then identified by RiboPrinter system could be an efficient method to detect Salmonella Ⅲ b 60：r：e，n，x，z15.