Abstract:Using osmanthus leaves as raw materials，the osmanthus leaf powder was soaked with a certain volume fraction of ethanol solution for a certain period of time，and a certain amount of hot water at a certain temperature was added to extract the polysaccharides.The effected of ethanol volume fraction，liquid-to-liquid ratio，extraction temperature and extraction time on the extraction of polysaccharides from osmanthus leaves were investigated.The results showed that the yield of polysaccharides reached 8.62%，when the ethanol volume fraction was 33%，the liquid-to-liquid ratio was 22∶1（mL/g），the extraction temperature was 70℃，and the extraction time was 8 min.The polysaccharide yield was high，and the experiment reproducibility was good.Osmanthus fragrans leaves polysaccharide has good antioxidant activity.