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投稿时间：2020-01-30
投稿时间：2020-01-30
中文摘要: 为探讨北虫草刺五加组合物（Cordyceps militaris Acanthopanax senticosus composition，CAC）抗疲劳作用及机制，采用转棒和力竭动物模型，将小鼠分为空白对照组和低、中、高剂量给药组，各组连续灌胃蒸馏水或不同剂量CAC，灌胃第29天，进行连续3 d的转棒适应实验，灌胃第32天进行正式转棒实验，测定小鼠转棒停留时间；灌胃第35天，进行负重力竭游泳实验，测定力竭时间以及小鼠血乳酸（lactic acid，LA）、血乳酸脱氢酶（lactate dehydrogenase，LDH）、血尿素氮（blood urea nitrogen，BUN）、肝糖原（liver cogen，LG）、肌糖原（muscle glycogen，MG）、肝组织中总超氧化物歧化酶（total superoxide dismutase，T-SOD）、丙二醛（malondialdehyde，MDA）、活性氧（reactive oxygen species，ROS）以及磷酸化-蛋白激酶B（phosphorylated-protein kinase B，p-AKT）、磷酸化-糖原合成酶激酶（phosphorylated-glycogen sythesis kinase-3，p-GSK3β）和葡萄糖转运子4（glucose transporter 4，GLUT4）等疲劳相关生化指标和糖原代谢相关机制指标。研究发现：与空白对照组相比，给药各组均可显著延长小鼠转棒的停留时间和力竭游泳时间（P<0.01），中、高剂量CAC可显著提高力竭小鼠体内T-SOD活性，降低MDA和ROS水平（P<0.01）；与空白对照组相比，中、高剂量CAC可极显著提高力竭小鼠LG、MG、LDH的含量和活性（P<0.001）、极显著降低LA、BUN的含量（P<0.001）；与空白对照组相比，中、高剂量CAC可增加肝组织中p-AKT（Ser473）、p-GSK3β（Ser9）和GLUT4蛋白的表达，其中高剂量组作用显著（P<0.01）。结果表明：CAC能够通过提高小鼠机体抗氧化活力和糖原储备，减少机体无氧酵解及蛋白质分解供能而发挥抗疲劳作用，该作用可能与激活AKT/GSK3β/GLUT4信号通路有关。
Abstract:The effect and mechanism of Cordyceps militaris Acanthopanax senticosus composition（CAC）for anti-fatigue were determined by rotating rod test and exhaustion animal model.The mice were divided into 4 groups，including the control group，low-dose CAC group，medium-dose CAC group and high-dose CAC group，which were given distilled water or various doses of CAC by gavage daily.Since the 29th day，Rotating rod test were performed for the mice to adapt them for three days.On the 32th day，the formal rotating rod test were performed after intragastric administration，and the residence time of the mice was determined.On the 35th day after intragastric administration，the weight-loaded swimming test were performed.The exhau-stive time，fatigue-related biochemical parameters and glycogen metabolism related mechanism indexes，including blood lactic acid（LA），lactate dehydrogenase（LDH），blood urea nitrogen（BUN），liver glycogen（LG），muscle glycogen（MG），total superoxide dismutase（T-SOD），malondialdehyde（MDA），reactive oxygen species（ROS），phosphorylated-protein kinase B（p-AKT），phosphorylated-glycogen sythesis kinase-3β（p-GSK3β）and glucose transporter 4（GLUT4）were determined.The results showed that compared with the control group，the duration of rotating rod test and the time of the weight-loaded swimming test were significantly prolonged in each group（P<0.01）.Compared to the control group，both in medium-dose CAC group and in highdose CAC group，the activities of T-SOD were significantly enhanced and the levels of MDA and ROS were decreased（P<0.01）.And compared with the control group，both in medium-dose CAC group and in high-dose CAC group，the content and activity of LG，MG and LDH were highly significant increased，and the content of LA and BUN were highly significant decreased （P<0.01）.And compared with the control group，both in medium-dose CAC group and in high-dose CAC group，the expression of p-AKT（Ser 473），p-GSK3β（Ser 9）and GLUT4 in livers were increased，especially in the high-dose group with significant effect（P<0.01）.The results showed that CAC could play an anti-fatigue role by increasing the antioxidant activity and glycogen reserve of mice，and reducing anaerobic glycolysis and energy supply from proteolysis，which may be related to activate the signal pathway of the AKT/GSK3β/GLUT4.
keywords: Cordyceps militaris Acanthopanax senticosus composition（CAC） anti-fatigue oxidative stress glycometabolism AKT signal transduction pathway
文章编号：202020003 中图分类号： 文献标志码：
基金项目:吉林省教育厅“十三五”科学技术项目（JJKH20170066KJ、JJKH20170067KJ）；吉林省科技厅省级医药健康产业发展专项资金项目（20170311039YY）；吉林省科技厅优秀青年人才基金项目（20180520051JH）
| Author Name | Affiliation |
| SHENG Yu，BAI Li-dan，DUAN Yi-han，AN Li-ping，GUO Xiao，WANG Man-li | College of Pharmacy，Beihua University，Jilin 132013，Jilin，China |
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