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投稿时间：2019-12-25
投稿时间：2019-12-25
中文摘要: 以南极磷虾肽（antarctic krill peptides，AKP）和FeCl2·4H2O为原料，以亚铁螯合率和螯合物得率为评价指标，在单因素试验基础上，利用正交试验优化制备出南极磷虾肽-亚铁螯合物（antarctic krill peptide-ferrous chelates，AKP-Fe），并对其理化性质进行分析。结果表明：AKP-Fe的最佳制备条件为：肽铁质量比3∶1，螯合温度40℃，螯合时间40 min，pH值5.5，肽浓度4%，乙醇体积倍数6。在此条件下测得亚铁螯合率为77.25%，螯合物得率为41.03%。傅里叶变换红外光谱分析显示AKP的氨基和羧基与Fe2+发生结合；扫描电镜分析显示AKP与Fe2+螯合后由松疏的片状结构变为颗粒状的聚集体。
Abstract:Antarctic krill peptides（AKP）and FeCl2·4H2O were used as the raw material.The effects of various factors on iron-chelating rate and chelate yield were investigated and the chelating process were optimized.Using one-factor-at-a-time method and orthogonal test，and the characterization of peptides-ferrous chelate was investigated.The optimal chelating conditions were obtained as follows that AKP-Fe dichloride mass ratio of 3∶1，chelating temperature of 40℃，chelating time of 40 min，pH value of 5.5，AKP concentration of 4%，ethanol volume ratio of 6.The actual iron-chelating rate under the optimized condition was 77.25%，chelate yield was 41.03%.Fourier transform infrared spectroscopy（FTIR）analysis suggested that the amino and carboxyl groups of AKP were chelated with Fe2+.Scanning electron microscopy（SEM）images showed that the microstructure of AKP changed dramatically after the chelation with Fe2+，turning from loose and sparse flake structure into granular aggregates.
keywords: antarctic krill peptides peptide-ferrous chelate preparation iron-chelating rate physicochemical properties
文章编号：202019016 中图分类号： 文献标志码：
基金项目:国家自然科学基金青年科学基金项目（41706166）
| 作者 | 单位 |
| 林海燕，王珊珊，马玉洁，吕世伟，周德庆 | 中国水产科学研究院黄海水产研究所，青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室，山东青岛266071；上海海洋大学食品学院，上海201306 |
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