Abstract:The effects of different technological parameters on the extraction rate of polyphenols from Ilex latifolia Thunb.were studied by response surface method.The technological conditions were optimized according to the experimental of Box-Behnken design.The total reducing power of polyphenols from Ilex latifolia Thunb.was measured by potassium ferricyanide reduction method，and the inhibitory activity of polyphenols from Ilex latifolia Thunb.on α-glucosidase and α-amylase was measured by spectrophotometer，and its antioxidant and hypoglycemic activities were preliminarily evaluated.Through single factor and orthogonal experiments，the optimum extraction conditions of polyphenols from Ilex latifolia Thunb.were determined as follows：extraction time 107 min，material liquid ratio 1∶21（g/mL），ultrasonic power 62 W，and the extraction rate of polyphenols from Ilex latifolia Thunb.was 54.13%.The results of antioxidant experiment showed that when the concentration of polyphenol was 0.02 mg/mL-1.0 mg/mL，the total reducing power increased rapidly with the increase of sample concentration，and the IC50value was 0.584 mg/mL.It was found that polyphenol from Ilex latifolia Thunb.had a certain inhibition on the activity of α-glucosidase and α-amylase，and its IC50values were 1.15 mg/mL and 1.31 mg/mL，respectively.The results showed that the polyphenols had good antioxidant and hypoglycemic activities，which had certain guiding significance for clinical treatment and the development of health food.