Abstract:52 accessions of Perilla frutescens（L.）Britton from different production areas were studied by using soxhlet extraction method，kjedahl method and anthrone colorimetric method.The results showed that the crude fat，the protein and the soluble sugar content of 52 accessions of Perilla frutescens（L.）Britton ranged from 35.06%to 53.28%，17.44%to 33.66%and 1.79%to 3.57%with an average of 45.09%，25.46%and 2.65%，respectively.There was difference among Perilla frutescens（L.）Britton from different production areas.There was the highest content of crude fat from seeds of Perilla frutescens（L.）Britton of Jinsha County，the following was Nayong County，Qianxi County，Dafang County，Zhijin County，Hezhang County and Qixingguan District，while the Perilla frutescens（L.）Britton from Weining County had the lowest content of crude fat.The order of the protein content was Hezhang County＞Qixingguan District＞Nayong County＞Dafang County＞Qianxi County＞Zhijin County＞Weining County＞Jinsha County，but the difference among them was not significant.There was the highest content of soluble sugar from seeds of Perilla frutescens（L.）Britton of Qixingguan District，the following was Hezhang County，Jinsha County，Dafang County，Weining County，Qianxi County，Zhijin County and Nayong County.The correlation analysis showed that the protein content and the crude fat content，the protein content and the soluble sugar as well as the crude fat content and the soluble sugar in Perilla frutescens（L.）Britton seeds were highly positive correlated，reaching extremely significant levels.