Abstract:Morchella esculenta is a rare edible medicinal fungus，with high nutritional value and medicinal efficacy.The extracellular polysaccharides of Morchella esculenta（MEP）was obtained after deep fermentation and the in vitro hyperglycemic and hypolipidemic activity of MEP were detected.The results showed that Morchella esculenta grew rapidly under deep fermentation，generally 5-6 days of fermentation could obtain a significant biomass.After de-protein，decolorization and dialysis，the MEP mixture was obtained.Via DEAE-cellulose 52 gel-column MEP was primarily neutral polysaccharides and contained a small amount of acidic polysaccharides.The inhibition activity MEP to α-glucoside and α-amylase was detected and reached 20%and 40%at high MEP concentration respectively.At the sametime，MEP showed a strong inhibitory rate at 30%on lipase activity at a concentration of 5 mg/mL.The binding efficiency of bile acid showed that MEP bind to sodium glycocholate and sodium taurocholate at the rate of 35%and 30%respectively.And finally，we tested the effect of MEP on the glucose diffusion rate which showed that MEP retards the diffusion of glucose under solution conditions.Collectively，the results of this study showed that MEP had strong blood sugar hypoglycemia and lipid-lowering activity，and was expected to be used as an auxiliary drug for the treatment of related diseases.