Abstract:Based on the determination of amino acid types and contents in jujube wine by automatic amino acid analyzer，the optimum determination parameters of jujube wine were explored with elution temperature，flow rate and buffer time as the objects.The storage temperature and time were also evaluated.Then，5 kinds of jujube wine were determined by the new established determination method.The results showed that the chromatographic peaks can be basically separated by setting parameters as temperature gradients of 42，60，93℃，flow rate of 33 mL/h，elution of buffer 1 and buffer 2 for 6 min and 12 min，respectively.In addition，the retention time of peaks was still within the error range after storage at-20℃for 60 h.Qualitative analysis showed that 14 kinds of amino acids could be identified in 5 kinds of jujube wine，including 4 kinds of essential amino acids，and the content of proline was the highest.Quantitative analysis showed that the total amino acids content in Yuanling jujube wine was the highest，reaching 283.74 μg/mL，while the essential amino acid content of mu jujube wine accounted for a higher proportion of total amino acids.Therefore，the automatic amino acid analyzer can better analyze the types and contents of amino acids in jujube wine，and then realize the evaluation of their protein quality，among them，the protein quality of mu jujube wine was better.