Abstract:Five honey varieties from Shennongjia forestry district in Hubei were assessed including physical and chemical indicators，contents of total phenolics and total flavonoid.Their antioxidant activities were determined by DPPH·and ABTS+free radical scavenging experiments.The results showed that the physical and chemical indicators of five honey varieties from Shennongjia forestry district were confromed with honey＜GH/T 18796-2012＞.The contents of total flavonoids ranged from （9.95±0.04）mg/100 g to （13.66±0.04）mg/100 g.The contents of total phenolic acids ranged from（20.69±0.03）mg/100 g to（31.71±0.07）mg/100 g.The five honey varieties were identificated DPPH·and ABTS+free radical scavenging capacity with IC50of（39.14±0.16）mg/mL to（106.63±0.38）and（48.48±0.22）mg/mL to（127.07±1.41）mg/mL.The total phenolics content was positively correlated with DPPH·and ABTS+free radical scavenging ability，and the correlation coefficients was 0.973 and 0.920，respectively.It was reported the physical and chemical indicators and antioxidant activities of honey in Shennongjia forestry district for the first time in this study，which provided a theoretical basis for the development and utilization of honey in Shennongjia forestry district.