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食品研究与开发:2020,41(13):191-197
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浓香型白酒窖泥中放线菌的发酵特性研究
(1.四川轻化工大学酿酒生物技术及应用四川省重点实验室,四川宜宾644000;2.四川国检检测有限责任公司,四川泸州646000;3.宜宾海关,四川宜宾644000)
Study on the Fermentation Characteristics of Actinomycetes in Pit Mud of Luzhou-flavor Liquor
(1.Liquor Making Biotechnology and Application Key Laboratory of Sichuan Province,Sichuan University of Science&Engineering,Yibin 644000,Sichuan,China;2.Sichuan National Inspection and Testing Co.,Ltd.,Luzhou 646000,Sichuan,China;3.Yibin Customs,Yibin 644000,Sichuan,China)
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投稿时间:2019-07-08    
中文摘要: 以从浓香型白酒窖泥所分离的白色链霉菌、桑氏链霉菌、鲁地链霉菌为试验对象,对其进行产酶能力研究、共酵试验以及固态发酵产酒试验,探究该放线菌在酿酒过程中的发酵特性。结果表明:放线菌具有较高的酶活力,可促进丁酸菌产酸;对白酒产香有积极作用,能够提高酯类、3-羟基-2-丁酮与2,3-丁二醇等香味物质含量。其中,桑氏链霉菌的发酵性能更好:用其制备的麸曲糖化酶活力为(661.8±10.2)U/g,蛋白酶活力(146.3±1.6)U/g,酯化力为(26.0±0.8)mg/(g·100 h),且能够显著提高香味物质含量。
中文关键词: 放线菌  窖泥  酶活力  共酵  固态发酵
Abstract:Taking Streptomyces albus,Streptomyces rutgersensis and Streptomyces sampsonii separated from pit mud of Luzhou-flavor liquor as experimental objects,the enzyme production ability,co-fermentation test and solid-state fermentation test were studied to explore the fermentation characteristics of the actinomycetes in liquor-making process.The results showed that actinomycetes had high enzyme activity and could promote acid production by butyric acid bacteria,and had positive effects on liquor aroma production,which could increased the content of esters,3-hydroxy-2-butanone and 2,3-butanediol.Among them,the fermentation performance of Streptomyces sampsonii was better,the glucoamylase activity of bran-qu prepared by Streptomyces sampsonii was(661.8±10.2)U/g,protease activity was(146.3±1.6)U/g,esterification ability was(26.0±0.8)mg/(g·100 h),and the content of aroma substances was significantly increased.
文章编号:202013031     中图分类号:    文献标志码:
基金项目:四川省科技厅应用基础研究项目(2019YJ0462);中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2018JJ007);酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2015-08,NJ2016-05);自贡市2019年科技创新苗子工程项目(2019CXMZ07)
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