Abstract:DPPH method and IC50of the half-value clearance of hydroxyl radical was used to optimize the extraction process of Mori Fructus antioxidant active components，and the antioxidant capacity of Mori Fructus in different place of origin，circulation areas，picking and purchase batches was evaluated.On the basis of single factor investigation of extraction method，extraction solvent，solid-liquid ratio and extraction time，orthogonal test was carried out according to L9（34）three-factor three-level table to optimize the extraction method of Mori Fructus active component group.The optimal extraction process was 0.3 g，20 mL 50%ethanol，30 min ultrasonic extraction，and the antioxidant activity IC50of each batch ranged from 0.041 to 0.456.