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食品研究与开发:2020,41(12):90-95
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响应面法优化微波辅助提取菜豆总黄酮工艺及对运动大鼠心肌线粒体的影响
(1.河池学院,广西河池547000;2.广西大学,广西南宁530004)
Optimization of Microwave-assisted Extraction of Total Flavonoids from Kidney Bean by Response Surface Methodology and Its Effect on Myocardial Mitochondria of Exercise Rats
(1.He Chi Universiry,Hechi 547000,Guangxi,China;2.Guangxi University,Nanning 530004,Guangxi,China)
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投稿时间:2019-06-05    
中文摘要: 通过响应面法优化微波辅助提取菜豆总黄酮工艺,并研究其对运动大鼠心肌线粒体的影响。结果表明:采用乙醇体积分数为61%、微波功率为355 W、微波温度为50℃时,进行3次平行试验,以验证工艺的稳定性,实际得出的菜豆总黄酮平均提取率为2.43%,与理论值相比误差较小,表明该方案适合用来提取菜豆总黄酮。同时对大鼠灌胃菜豆总黄酮表明其具有良好的抗氧化能力,并起到清除自由基的作用,进而对心肌线粒体起到保护作用。
Abstract:Response surface methodology was used to optimize the microwave-assisted extraction of total flavonoids from kidney bean and to study its effect on myocardial mitochondria of exercise rats.The results showed that when the volume fraction of ethanol was 61%,the microwave power was 355 W,and the microwave temperature was 50℃,three parallel experiments were carried out to verify the stability of the process.The average extraction rate of total flavonoids in bean was 2.43%,which was less error compared with the theoretical value,indicated that the scheme was suitable for extracting total flavonoids in bean.At the same time,the total flavonoids of kidney bean showed that it had good antioxidant capacity,and played a role in scavenging free radicals,and then played a protective role in myocardial mitochondria.
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